Biochemistry and Biophysics of Macromolecules and Molecular Assemblies
9 units (3- 0-6) | first term
Prerequisites: Bi/Ch 110. Detailed analysis of the structures of the four classes of biological molecules and the forces that shape them. Introduction to molecular biological and visualization techniques.
9 units (3-0-6) | second term
Basic principles of modern biophysical and structural methods used to interrogate macromolecules from the atomic to cellular levels, including light and electron microscopy, X-ray crystallography, NMR spectroscopy, single molecule techniques, circular dichroism, surface plasmon resonance, mass spectrometry, and molecular dynamics and systems biological simulations.
Instructors: Clemons, Jensen, and other guest lecturers
Molecular Machines in the Cell
9 units (3-0-6) | third term
Prerequisites: Bi/Ch 110, BMB/Bi/Ch 170, BMB/Bi/Ch 173.
Detailed analysis of specific macromolecular machines and systems that illustrate the principles and biophysical methods taught in BMB/Bi/Ch 170 and BMB/Bi/Ch 173.
Instructors: Clemons, Hoelz, Shan and various guest lecturers (subject to change each year)
Macromolecular Function: Kinetics, Energetics, and Mechanisms
9 units (3-0-6) | first term
Prerequisites: Bi/Ch 110 or equivalent.
Discussion of the energetic principles and molecular mechanisms that underlie enzyme's catalytic proficiency and exquisite specificity. Principles of allosteric regulation, selectivity, enzyme evolution, and computational enzyme design. Practical kinetics sections discuss how to infer molecular mechanisms from rate/equilibrium measurements and their application to more complex biological systems, including steady-state and pre-steady-state kinetics, kinetic simulations, and kinetics at the single molecule resolution.
BMB/Ch 202 abc
Biochemistry Seminar Course
1 unit | first, second, third terms
A course that includes a seminar on selected topics from outside faculty on recent advances in biochemistry. Students will participate in the seminar along with a formal discussion section with visiting faculty. Students will meet with the Biochemistry seminar speaker in the discussion section for an hour, and then attend the Biochemistry seminar at 4 p.m. BMB Seminars take place 1-2 times per month (usually on Thursdays).
Macromolecular Structure Determination with Modern X-ray Crystallography Methods
12 units (2-4-6) | third term
Prerequisites: BMB/Bi/Ch 170 and consent of instructor.
Advanced course in macromolecular crystallography integrating lecture and laboratory treatment of diffraction theory, crystallization (proteins, nucleic acids and macromolecular complexes), crystal characterization, X-ray sources and optics, crystal freezing, X-ray diffraction data collection (in-house and synchrotron), data reduction, multiple isomorphous replacement, single- and multi-wavelength anomalous diffraction phasing techniques, molecular replacement, electron density interpretation, structure refinement, structure validation, coordinate deposition and structure presentation. In the laboratory component, one or more proteins will be crystallized and the structure(s) determined by several methods, in parallel with lectures on the theory and discussions of the techniques
Bi/BMB 251 abc
Current Research in Cellular and Molecular Biology
Prerequisites: graduate standing.
Presentations and discussion of research at Caltech in biology and chemistry. Discussions of responsible conduct of research are included.
Instructors: Sternberg, Hay
Fundamentals of Molecular Genetics
9 units (3-0-6) | third term
Principles and mechanisms of DNA repair and replication, transcription and splicing, and protein synthesis. Not offered 2016-17.
Units to be arranged | first, second, third terms
Students may register for research units after consultation with their adviser.
Published Date: July 28, 2022